醫(yī)學(xué)論文范文:川芎嗪對(duì)谷氨酸損傷海馬神經(jīng)元的保護(hù)作用

醫(yī)學(xué)論文范文:川芎嗪對(duì)谷氨酸損傷海馬神經(jīng)元的保護(hù)作用

【摘要】 目的 觀察川芎嗪對(duì)谷氨酸損傷海馬神經(jīng)元的保護(hù)作用。方法 原代培養(yǎng)大鼠海馬神經(jīng)元，培養(yǎng)基中加入1mmol/L的鹽酸川芎嗪溶液共同孵育12h后，加入1mmol/L谷氨酸損傷海馬神經(jīng)元20min。采用MTT法檢測(cè)細(xì)胞活性;測(cè)定培養(yǎng)液中乳酸脫氫酶(LDH )活力的變化;細(xì)胞免疫組化檢測(cè)各組細(xì)胞表達(dá)AchE。 結(jié)果 川芎嗪可提高谷氨酸損傷后海馬神經(jīng)元的存活率，抑制因谷氨酸損傷引起的細(xì)胞內(nèi)LDH的釋放，可促進(jìn)谷氨酸損傷細(xì)胞中AChE的表達(dá)。 結(jié)論 川芎嗪對(duì)谷氨酸誘導(dǎo)海馬神經(jīng)元損傷有保護(hù)作用。

【關(guān)鍵詞】 川芎嗪;谷氨酸;海馬神經(jīng)元

Protective effects of ligustrazine on glutamate�瞚nduced

neurotoxicity in cultured hippocampal neuronsBAO Zhi�瞛un, XIA Chun�瞝in

(Department of Anatomy and Cytoneurobiology, Medical School of

Soochow University, Suzhou 215123, China)ABSTRACT: Objective To observe the protective effects of ligustrazine on glutamate�瞚nduced injury in cultured hippocampal neurons. Methods Primarily cultured hippocampal neurons from fetal rats were incubated with ligustrazine (1mmol/L) for 12 hours, then glutamate (1mmol/L) was added for 20 minutes to induce injury. Cell viability was detected by MTT assay, and the change in LDH activity was determined by biochemical method. Finally, the expression of AchE in the cultured neurons was assayed by immuocytochemistry. Results Ligustrazine improved the survival rate of hippocampal neurons injured by glutamate, inhibited the release of LDH from the kytoplasm injured by glutamate. The expression of AchE in the injured neurons was promoted by pretreatment of ligustrazine. Conclusion Ligustrazine can significantly exert protective effects on the hippocampal neuron injury induced by glutamate.

KEY WORDS: ligustrazine; glutamate; hippocampal neuron醫(yī).學(xué)全.在.線網(wǎng)站bhskgw.cn

海馬是邊緣系統(tǒng)的重要組成部分，參與學(xué)習(xí)、記憶、情緒反應(yīng)及植物神經(jīng)功能等。原代培養(yǎng)的海馬神經(jīng)元對(duì)許多致病因素和化學(xué)損害較為敏感，是研究神經(jīng)元損傷及抗損傷的理想模型。在腦缺血模型中，海馬神經(jīng)元的損傷較為嚴(yán)重，其中興奮性氨基酸含量增高是海馬神經(jīng)元損傷的原因之一。谷氨酸(glutamate, Glu)是哺乳動(dòng)物腦內(nèi)主要的興奮性氨基酸，參與中樞神經(jīng)系統(tǒng)的信息傳遞。但Glu過(guò)度釋放可產(chǎn)生神經(jīng)興奮性毒性，引起神經(jīng)細(xì)胞損傷或死亡。川芎嗪(ligustrazine, Lig)是從中藥川芎總生物堿中分離得到的一種有效活性生物堿。Lig能通過(guò)血腦屏障進(jìn)入中樞神經(jīng)系統(tǒng)，在腦中快速達(dá)到峰值，并持久地存留在大腦內(nèi)。臨床上常用Lig治療腦缺血，其機(jī)制是通過(guò)擴(kuò)張微血管改善微循環(huán)，抑制血栓形成，阻斷自由基連鎖反應(yīng)，降低細(xì)胞內(nèi)鈣離子超載，拮抗氨基酸毒性等途徑來(lái)實(shí)現(xiàn)，具有多靶點(diǎn)、多層次、多環(huán)節(jié)的腦保護(hù)作用[1]。本研究采用體外海馬神經(jīng)元原代培養(yǎng)，觀察Lig對(duì)Glu損傷海馬神經(jīng)元的保護(hù)作用，探討Lig對(duì)腦損傷神經(jīng)元的保護(hù)機(jī)制。

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