醫(yī)學(xué)免費(fèi)論文:異常黑膽質(zhì)證載體大鼠模型的建立及其免疫、內(nèi)分泌紊亂狀態(tài)的研究

醫(yī)學(xué)免費(fèi)論文:異常黑膽質(zhì)證載體大鼠模型的建立及其免疫、內(nèi)分泌紊亂狀態(tài)的研究

【摘要】 目的建立維吾爾醫(yī)異常黑膽質(zhì)證載體大鼠模型，并探討其免疫、內(nèi)分泌的紊亂狀態(tài)。方法雄性Wistar大鼠20只, 穩(wěn)定飼養(yǎng)3 d后隨機(jī)分為正常對照組(10只)和模型組(10只),其中模型組根據(jù)維吾爾醫(yī)學(xué)理論，采用干寒飼養(yǎng)環(huán)境、干寒屬性的飼料、慢性間斷足底電刺激、強(qiáng)迫游泳、制動等多因素復(fù)合作用3周，建立維吾爾醫(yī)異常黑膽質(zhì)證載體大鼠模型，動態(tài)觀察模型組大鼠一般狀態(tài)、體重、飲食量、飲水量和舌苔的變化;檢測外周血白細(xì)胞介素1β(IL��1β)、白細(xì)胞介素6(IL��6)、腫瘤壞死因子α(TNF�拨�)、促腎上腺皮質(zhì)激素(ACTH)和皮質(zhì)醇(CORT)等免疫、內(nèi)分泌指標(biāo)的變化。結(jié)果與正常對照組相比，模型組動物的一般狀態(tài)與異常黑膽質(zhì)證候特征相一致。第11天至第21天體重增長速度慢，自第7天至第21天飲食量、飲水量增多，差異有統(tǒng)計學(xué)意義(P<0.01)。與正常對照組相比，模型組動物血清ACTH、CORT、IL��1β水平均降低，差異有統(tǒng)計學(xué)意義(P<0.05~0.01),IL��6水平降低，但差異無統(tǒng)計學(xué)意義(P>0.05),TNF�拨了�平升高,差異有統(tǒng)計學(xué)意義(P<0.05)。結(jié)論異常黑膽質(zhì)證載體大鼠模型的一般狀態(tài)、體重、飲食量、飲水量的變化與臨床患者以及小鼠動物模型中異常黑膽質(zhì)證相似，并且其免疫、內(nèi)分泌處于紊亂狀態(tài)。

【關(guān)鍵詞】 異常黑膽質(zhì); 維吾爾醫(yī)學(xué); 動物模型

Establishment of abnormal savda syndrome rat model and study on its disordered immune�瞖ndocrine network

ZHANG You�瞙ui, Halmurat·Upur, Yusup·Tursun, et al醫(yī).學(xué).全.在.線bhskgw.cn

(Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, Urumqi 830011, China)

Abstract: ObjectiveTo establish a rat model of abnormal savda syndrome in Uygur medicine and investigate its disordered immune�瞖ndocrine network. MethodsMale Wistar rats (n=20)， were randomly divided into control group (n=10) and model group (n=10) after stable feeding 3 d. According to the theory of Uygur medicine, model group rats were fed with cold�瞡atured diet under cool dry environment, stimulated with chronics and interrupted electric foot shocks, enforced swimming and controlled movement to establish abnormal savda syndrome rat model for three weeks, rats′ behavior, body weight, daily food intake, daily water intake, state of tongue were observed. The concentrations of immune�瞖ndocrine related indexes including Interleukin��1β (IL��1β), Interleukin��6 (IL��6), Tumor necrosis factor�拨� (TNF�拨�), Adrenocorticotropic Hormone (ACTH) and Cortisol (CORT) in serum were measured by enzyme linked immunosorbent assay (ELISA) method. ResultsCompared with the control group, the model group developed abnormal savda syndrome like slow weight gaining, more diet, more drink. Compared with the control group, the serum level of ACTH， CORT， IL��1β in model group decreased，and the differences had statistical significance (P<0.05~ 0.01); the serum level of IL��6 in model group decreased，but the differences had no statistical significance (P>0.05); the serum level of TNF�拨� increased, and the differences had statistical significance (P<0.05). ConclusionThe changes of body weight, daily food intake, daily water intake of abnormal savda syndrome rat model are similar to the feature of human abnormal savda syndrome and the abnormal savda syndrome model of ICR mice. Abnormal savda syndrome rat model developes disordered immune�瞖ndocrine network.

Key words: abnormal savda; Uygur medicine; animal model

維吾爾醫(yī)學(xué)認(rèn)為異常黑膽質(zhì)是在各種內(nèi)外因素的作用下，體液質(zhì)平衡紊亂，體液質(zhì)被燃燒，繼而沉淀的最終病理產(chǎn)物或表現(xiàn)形式。長期受到慢性刺激、進(jìn)食干寒屬性的飼料、處于干寒的環(huán)境是異常黑膽質(zhì)證發(fā)生的主要起因[1-2]。隨著維吾爾醫(yī)學(xué)的深入研究,需要建立具有維吾爾醫(yī)學(xué)證候特征的大鼠模型,以便運(yùn)用現(xiàn)代科學(xué)手段研究維吾爾醫(yī)學(xué)。本研究以維吾爾醫(yī)學(xué)理論為指導(dǎo)，采用干寒飼養(yǎng)環(huán)境、干寒屬性的飼料、慢性間斷足底電刺激、強(qiáng)迫游泳和制動等多因素復(fù)合作用3周，建立維吾爾醫(yī)異常黑膽質(zhì)證載體大鼠模型，并檢測其外周血白細(xì)胞介素1β(IL��1β)、白細(xì)胞介素6(IL��6)、腫瘤壞死因子α(TNF�拨�)、促腎上腺皮質(zhì)激素(ACTH)和皮質(zhì)醇(CORT)等免疫、內(nèi)分泌指標(biāo)的變化，探討其免疫、內(nèi)分泌的紊亂狀態(tài)。

1　材料與方法

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